Thiết kế mồi gene cho phản ứng Nested-MSP khuếch đại gene RASSF1A (Ras association domain family member 1)

نویسندگان

چکیده

Tính chất methyl hóa các gene ức chế khối u là một trong những nguyên nhân gây nên ung thư vòm họng. RASSF1A đóng vai trò quan trọng điều hòa chu kì tế bào, chỉnh sự ổn định của vi ống và có khả năng kiểm soát xâm lấn di căn. Trong nghiên cứu, cặp mồi sử dụng cho phương pháp Nested-MSP sẽ được thiết kế dựa trên công cụ tin sinh học: Methprimer, IDT Annhyb. Kết quả chúng tôi đã thành mồi, bao gồm: ngoài, unmethyl phản ứng khuếch đại sản phẩm PCR với kích thước lần lượt 392bp, 200bp 173bp. Như vậy, từ cứu này cung cấp hướng tới khảo sát tính Ung Thư Vòm Họng (UTVH).

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Epigenetic inactivation of RAS association domain family protein 1 (RASSF1A) in malignant cutaneous melanoma.

Recent findings have shown the inactivation of a Ras effector homologue gene referred to as the Ras association domain family 1 (RASSF1) gene, which is a potential human tumor suppressor gene located on chromosome 3p21.3. Hypermethylation of the CpG island promoter region of a major alternative transcript of this gene, RASSF1A, has been suggested to play a key role in pathogenesis of various ca...

متن کامل

Identification of novel gene expression targets for the Ras association domain family 1 (RASSF1A) tumor suppressor gene in non-small cell lung cancer and neuroblastoma.

RASSF1A is a recently identified 3p21.3 tumor suppressor gene. The high frequency of epigenetic inactivation of this gene in a wide range of human sporadic cancers including non-small cell lung cancer (NSCLC) and neuroblastoma suggests that RASSF1A inactivation is important for tumor development. Although little is known about the function of RASSF1A, preliminary data suggests that it may have ...

متن کامل

Role of the Ras-association domain family 1 tumor suppressor gene in human cancers.

In recent years, the list of tumor suppressor genes (or candidate TSG) that are inactivated frequently by epigenetic events rather than classic mutation/deletion events has been growing. Unlike mutational inactivation, methylation is reversible and demethylating agents and inhibitors of histone deacetylases are being used in clinical trails. Highly sensitive and quantitative assays have been de...

متن کامل

RHOA ( ras homolog gene family , member A ) Teresa

RhoA encodes a 21-kDa, 193 amino acids, small Rho GTPase; it displays potent oncogenic activity when overexpressed. RhoA structure: The N-terminal half of RhoA contains the majority of the amino acids involved in GTP binding and hydrolysis, together with the Switch 1 and 2 regions that change conformation between the GTP-bound and GDP-bound states. Several X-ray crystallographic structures of R...

متن کامل

the association of ala133ser polymorphism and methylation in ras association domain family 1a gene with unfavorable prognosis of hepatocellular carcinoma

conclusions the polymorphism and promoter methylation of rassf1a may be a significant factor in hcc, and can be an indicator for poor prognosis in patients with hcc. background the functional and prognostic significance of ras association domain family 1a gene (rassf1a) on hepatocellular carcinoma (hcc) has not been well characterized. objectives this study aimed to investigate the association ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Ta?p chi? Khoa ho?c ?a?i ho?c M?? Tha?nh phô? Hô? Chi? Minh - Ky? thuâ?t va? Công nghê?

سال: 2023

ISSN: ['2734-9594', '2734-9322']

DOI: https://doi.org/10.46223/hcmcoujs.tech.vi.18.1.2023.2023